Training and webinars

The training component of EURIPRED, comprising of workshops and laboratory exchanges has created opportunities for scientists from candidate countries, new member states, and also from the developing countries, to become integrated into our project. Trainings have taken place at several key laboratories, but have also included e-learning modules. 

The following trainings and webinars are currently available: 

1. !!NEW (July 2017)!! Video tutorial: Antigen Formats for Antigen-Specific T-Cell Assays. The tutorial is provided by JPT Peptide Technologies in Berlin and can be found here.

Main topics:
- General comments on antigens
- Use of antigens in T-cell assays
- Advantages and disadvantages
- Controls
- TERS

Target group: scientists involved in cellular immune response monitoring, T-cell assay development, T-cell epitope mapping, biomarker discovery, target antigen discovery etc...

2. Video tutorial: Peptide Library Generation. The tutorial is provided by JPT Peptide Technologies in Berlin. 

Main topics:        
- principles of library generation
- peptide libraries to display entire protein sequences in resepect to isoforms, variations and homologue sequences
- optimising libraries concerning overlap, amino acid-coverage
-optimising concerning homologue protein sequences, protein coverage

Target group: scientists involved in library design, biomarker discovery, antigen/protein definition, etc. 

3. Video tutorial: Microarray Technology for Humoral Immune Monitoring. The tutorial is provided by  JPT Peptide Technologies in Berlin

Main topics:
- overview on technology
- requirements for using microarray technology
- assay workflow and data managment
- examples of different evaluations

Target group
: scientists involved in humoral immune response monitoring, seromarker discovery, B-cell epitope mapping, target antigen discovery, etc. 

 

The following trainings and webinars have taken place in the past:

1. March 2017On-site lab course at Helmholtz Centre for Infection Research: Mucosal vaccination strategies and immunomonitoring focusing on FACS analysis (multifunctional T cells and intracellular cytokines). Target group for the course: Scientists performing animal studies for developing vaccine related technologies or preclinical validation of vaccine candidates

2. October 2015 and March 2016: Characterization of humoral and cellular immune responses following mucosal vaccination: two on-site lab courses (five-day hands-on training) for up to 6 participants at the labs of the research group vaccinology at the Helmholtz Centre for Infection Research (HZI) in Braunschweig, Germany. Lab course I:  Mucosal vaccination strategies and immunomonitoring and Lab course II:  Multifunctional T cells and intracellular cytokines (read the agenda)
Target group for both courses: Scientists performing animal studies for developing vaccine related technologies or preclinical validation of vaccine candidates. More information.
More information here.

3. October 2015: Obtaining HIV primary isolates: Biological and genetic characterization of HIV-1 strains: on-site training course, consisting of a 2 week practical course for up to 2 participants, which will take place at the HIV Biology and Variability Unit, National Centre for Microbiology, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain. 

Contents of the training: a) HIV primary isolation from patients’ PBMCs, by coculture with PBMCs from healthy donor, previously stimulated, in accordance with the AIDS Clinical Trial Group (ACTG) VRL protocol. B) Coreceptor (CCR5 and/or CXCR4) usage determination of HIV primary isolates by infecting GHOST-CCR5 and GHOST-CXCR4 cells. c) Near full-length genome sequencing of HIV primary isolates d) Phylogenetic sequence analysis by maximum likelihood and analysis of recombination by bootscanning.

Target group: a) Scientists interested in the availability of well characterized HIV isolates for vaccine candidate assessment, for neutralization studies to assess the humoral immune responses and for studies on HIV molecular diagnostic techniques and b) Scientists interested in the use of methods for phylogenetic HIV sequence analysis and characterization of HIV genetic forms.

4. April 2015: A practical course for immunologists on high throughput seroprofiling and antibody epitope mapping using peptide microarrays has been organized by JPT Peptide Technologies in Berlin to learn how to get robust and reliable scientific results, easily and quickly. The course included hands-on laboratory sessions, using JPT’s RepliTope™ and PepStar™ peptide microrrays and an introduction to evaluation and presentation of complex data using R. Participants got tutorials, tips and tricks and presentations by experienced peptide microarray specialists from JPT.

Free services »

Reference reagents / adjuvant & formulation studies / microarray facility

 

Contact

Dr Mei Mei Ho
Project coordinator
+44 (0) 1707 641473
mei.ho(at)nibsc.org