SOPs

Biological and clinical materials, standards and data from several EURIPRED partners will be maintained, stored and made available to researchers worldwide to perform collaborative experiments. The availability and use of a large and extensive resource for Poverty Related Diseases in Europe will enable new developments in the vaccine field. Reference materials for developing immune assays will be available, along with malaria reference sear for diagnostics development and epidemiology studies. This will involve the organization of common standard operating procedures (SOPs), work practices, ethics and to coordinate the transfer of samples and clinical/laboratory information.
Newly developed SOPs will be published on this webpage during the course of the project.

  1. Protocol for HIV-1 near full-length genome amplification and sequencing from plasma RNA. More information can be obtained from Miguel Thomson, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda (Madrid)
  2. Table with sequences of primers used in protocol 1. More information can be obtained from Miguel Thomson, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda (Madrid)
  3. Protocol for construction of HIV-1 functional envelope clones. More information can be obtained from Miguel Thomson, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda (Madrid)
  4. Protocol for HIV-1 isolation from infected peripheral blood mononuclear cells. More information can be obtained from Lucía Pérez Álvarez, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda (Madrid)
  5. SOP for Taking blood at and receiving blood from TCC May 2010. More information can be obtained from the Biobank of King's College, London.
  6. SOP for Tracking of infected blood May 2010 More information can be obtained from the Biobank of King's College, London.
  7. SOP for Labelling samples stored in the BioBank May 2010 More information can be obtained from the Biobank of King's College, London.
  8. SOP for Spillage leakage May 2014 More information can be obtained from the Biobank of King's College, London.
  9. SOP Fractionating blood into sera, plasma and whole blood cell pellets from HIV infected Samples More information can be obtained from the Biobank of King's College, London.
  10. SOP Storage of blood fractions (plasma, sera, blood cells and granulocyte-rich fractions) derived from infected blood More information can be obtained from the Biobank of King's College, London.
  11. SOP Procedure for the Isolation of PBMCs from HIV infected blood More information can be obtained from the Biobank of King's College, London.

 

 

Free services »

Reference reagents / adjuvant & formulation studies / microarray facility

 

Contact

Dr Mei Mei Ho
Project coordinator
+44 (0) 1707 641473
mei.ho(at)nibsc.org